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Once the needle was positioned, antisense oligonucleotide was given in a volume of 5 microliters in saline vehicle and injected into the right (or left) lateral ventricle over 20-30 seconds. The needle was left in place for 10 seconds before removal. The needle tip was then inserted through the scalp and the skull, about 1 mm lateral and 1 mm caudal to bregma. A sterilized IT catheter, sexy naked females made of polyethylene tubing (PE10 tubing stretched to approximately 1.3 mm outer diameter), was inserted through the incision and carefully advanced caudally through the subarachnoid space while it was rotated between thumb and forefinger and while the base of the tail was gently pulled to align the spinal cord using the other hand. Using retractors or forceps, the muscle was peeled caudally to expose the cisternal membrane at the base of the skull. Once the catheter had been advanced to the desired area, it was flushed with 20 .mu.L sterile saline and the cranial end was passed through the skin using a 19 gauge needle about 1 cm from the incision.

A brief saline flush of 5 ul was given just prior to the antisense oligonucleotide delivery and a 10 .mu.l saline flush was given just following the oligonucleotide delivery at a rate of 10 .mu.l/minute to cover the dead volume of the catheter (6-7 .mu.l). Sequence score is a mathematical calculation determined for all oligomers and is based on the percent of G and C nucleotides, or analogs thereof, within a given oligomer sequence. The Sequence score of each oligomer was calculated to predict the suitability of the oligomers. The in vivo tolerability of the oligomers was tested to see how the oligomer was tolerated when injected into an animal. In vivo tolerability of the oligomers were tested in mice and rats. Mice were anesthetized using isoflurane anesthetic (1.5-4%). The mouse to be injected, weighing 20-30 g, was held by the loose skin at the back of the neck with the thumb and first fingers of one hand. Short term recognition memory was measured using the novel object recognition (NOR) task.

One hour later, mice were placed back into the test chamber for a 10 minute test session, this time with one object they had observed during training and one novel object. NOR testing was based on the spontaneous behavior of rodents to explore a novel object more than a familiar one (Dodart et. But whatever you think of the actual performance that followed, one thing it most definitely failed to provide was unity. For one hour following the single injection of antisense oligonucleotide ICV or IT, animals were observed for behavioral side effects and scored for the severity of side effects on a scale of zero (no side effects) to 20 (convulsions resulting in euthanasia). On the test day, animals were placed in the rectangular test chamber and allowed to explore two identical objects (7.6 cm high x 5.1 cm wide) for a 15 minute training period. Objects were cleaned thoroughly with 25% ethanol between training and testing sessions and between subjects, and were cleaned again at the end of the day with mild detergent. Richard Boath, now 61, was at the sharp end of the Qatari transaction, and tapes of him expressing concerns about the deal were the key to the prosecution case.

The torrent has also addressed security concerns that were brought up by admins and moderators last year, according to TorrentFreak. A 5-10 .mu.L volume of antisense oligonucleotide was injected slowly (over approximately 60 seconds) into the subarachnoid space. At least five days after surgery, a single antisense oligonucleotide injection was diluted in water and delivered via a programmable infusion pump (Knopf) at a rate of 10 .mu.l/minute in a volume of 10 to 50 .mu.l. After celebrating weddings in Cancun, newlyweds can extend the occasion with a romantic honeymoons where they can enjoy water sports activities in Nichupte Lagoon or explore the historic Mayan archaeological sites including Kinichna, Dzibanche and Tulum. All animals had ad libitum access to food and water throughout the studies. Animals for Tau qPCR and behavioral studies were adult, C57B1/6J female mice (20-30 g; Jackson Laboratories, Bar Harbor, Me.) housed 3-4 per cage. Each category was scored on a scale of 0-4, with the worst possible total score of 20. Animals were observed for changes in behavior in the home cage, and then they were removed from the home cage for more detailed observations which included measurement of grip strength and righting reflex. The sequence score of the selected oligomers were calculated for further studies.